Microbiology Lab Manual for Face-to-Face Labs
This is an online, free lab manual for the student in BIOL 2310L.
Lab Units
Unit 1
Microbiology Computer Use and Lab Safety
Unit 2
Microscope Use and Cell Length Calculations
Unit 3
Microbial Growth, Aseptic Inoculation & Streak Isolation
- Sterilization of an Inoculating Tool using a Microincinerator (Video)
- Sterilization of an Inoculating Tool using a Bunsen Burner (Video)
- Transferring Organism from a Slant Medium to a Broth Medium (Video)
- Transferring Organism from a Petri dish to a Slant (Video)
- Transferring Organism from a Broth to a Deep (Video)
- Creating a Lawn Petri Plate (Environmental Sample Example) (Video)
- Inoculating a Petri Plate with the Streak Isolation Technique (Video)
- Carbohydrate Fermentation - Streak Isolation Results (Video)
Unit 4
Streak Isolation from a Mixed Broth and Simple Stains
Unit 5
Differential Staining Technique: Gram Stain (Fall/Spring Term)
Differential Staining Technique: Gram Stain (Summer Term)
Unit 6
Unit 7
Supportive Selective and Differential Media
Unit 8
Unit 9
Unit 10
Unit 11
Unit 12
Susceptibility of Bacteria to Antibiotics and Introduction to Bacterial Identification
- Novobiocin (Video)
- A/P Disks (Video) (Note: This video uses a CNA blood plate but we will use the TSA with blood.)
Unit 13
Identification of Unknown Bacteria
Unit 14
Chemical Agents that Inhibit Bacterial Growth
Unit 15
Bacteriologic Analysis of Milk
Gram(+)/Gram(-) Results
Micro Biology Lab Manual by Karen Z. Bentz, Heather Fitzgerald, Patricia G. Wilber is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.